Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Blood Studies for Cardiovascular System I: Cardiac Biomarkers

Blood Studies for Cardiovascular System I: Cardiac Biomarkers

Cardiac biomarkers are enzymes, proteins, and hormones released into the blood when cardiac cells are injured. They are powerful tools for triaging.
The essential diagnostic tools for detecting myocardial necrosis and monitoring individuals suspected of having acute coronary syndrome (ACS) include:
Troponins
Troponins, particularly cardiac troponins I and T, are the most precise and sensitive markers of myocardial injury. They are detectable within 4-6 hours of myocardial injury and remain...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Effects of a clinical metagenomics intervention on clinical outcomes, healthcare costs, and health-related quality of life in patients with sepsis or septic shock: results of the randomized-controlled DigiSep trial.

Intensive care medicine·2026

Same author

Broad-Spectrum Antiviral and Antibacterial Activity of the Scorpion Venom Peptide HP1090.

Toxins·2026

Same author

Neuroglial P2Y<sub>1</sub> receptor signalling differentially contributes to inflammatory neurodegeneration.

Journal of neuroinflammation·2026

Same author

Impact of noradrenaline, adrenergic receptors, and radical formation on cardiac mitochondria in resuscitated swine.

Intensive care medicine experimental·2026

Same author

DHODH Inhibitors Based on the Vidofludimus Scaffold Containing Carboxylic Acid Bioisosteres Exert a Superior Broad-Spectrum Antiviral Activity.

Journal of medicinal chemistry·2026

Same author

The Cellular Response Capacity as Diagnostic Head Start in Neutrophil Endotoxemia Sensing.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2026

Search research articles

Related Experiment Video

Updated: Jan 6, 2026

Screening Assays to Characterize Novel Endothelial Regulators Involved in the Inflammatory Response

Screening Assays to Characterize Novel Endothelial Regulators Involved in the Inflammatory Response

Published on: September 15, 2017

Bioassay for Endothelial Damage Mediators Retrieved by Hemoadsorption.

Maximilian Denzinger¹, Ludger Staendker², Keno Ehlers¹

¹Division of Experimental Anesthesiology, University Hospital Ulm, Albert-Einstein-Allee 23, 89081, Ulm, Germany.

Scientific Reports

|October 12, 2019

Summary

Hemoadsorption devices remove harmful substances in septic shock. This study identified specific molecular patterns, including nucleic acids bound to proteins, that damage endothelial cells, suggesting hemoadsorption can protect these cells.

More Related Videos

Assessing Endothelial Vasodilator Function with the Endo-PAT 2000

Assessing Endothelial Vasodilator Function with the Endo-PAT 2000

Published on: October 15, 2010

A Simple Bioassay for the Evaluation of Vascular Endothelial Growth Factors

A Simple Bioassay for the Evaluation of Vascular Endothelial Growth Factors

Published on: March 15, 2016

Related Experiment Videos

Last Updated: Jan 6, 2026

Screening Assays to Characterize Novel Endothelial Regulators Involved in the Inflammatory Response

Screening Assays to Characterize Novel Endothelial Regulators Involved in the Inflammatory Response

Published on: September 15, 2017

Assessing Endothelial Vasodilator Function with the Endo-PAT 2000

Assessing Endothelial Vasodilator Function with the Endo-PAT 2000

Published on: October 15, 2010

A Simple Bioassay for the Evaluation of Vascular Endothelial Growth Factors

A Simple Bioassay for the Evaluation of Vascular Endothelial Growth Factors

Published on: March 15, 2016

Area of Science:

Biomedical Engineering
Critical Care Medicine
Molecular Biology

Background:

Septic shock involves inflammatory cytokines and molecular patterns damaging microvascular endothelial cells (mEC).
Hemoadsorption aims to remove these damaging substances, ideally restoring mEC function and patient recovery.
Current understanding of specific endothelial damage mediators adsorbed by hemoadsorption devices is incomplete.

Purpose of the Study:

To prove that endothelial-specific damage mediators can be adsorbed and recovered from hemoadsorption devices.
To identify molecular components within hemoadsorber fractions responsible for endothelial cell damage.
To establish a novel endothelial bioassay for evaluating hemoadsorption efficacy.

Main Methods:

Utilized a hemoadsorption device with polystyrene-divinylbenzene core particles.
Fractionated proteins eluted from the device.
Assessed effects of fractions on mEC proliferation, cell death, apoptosis, and ATP levels using video-based imaging and biochemical assays.

Main Results:

Identified 3 out of 39 fractions that inhibited mEC proliferation, increased cell death, and induced apoptosis.
These damaging fractions contained ~15 kDa proteins and significant amounts of oxidized nucleic acids.
Reduced ATP content in mEC was observed upon exposure to these fractions.

Conclusions:

Hemoadsorption devices can effectively capture endothelial damage-mediating molecules.
Circulating nucleic acid-protein complexes may act as danger-associated molecular patterns (DAMPs) exacerbating septic shock inflammation.
Hemoadsorption shows potential in limiting endothelial damage and improving barrier function by binding these DAMPs.