Related Experiment Video
Updated: Jan 6, 2026

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
Published on: October 2, 2021
Double Interferometer Design for Independent Wavefront Manipulation in Spectral Domain Optical Coherence Tomography
Jonas Kanngiesser1, Maik Rahlves2, Bernhard Roth2,3
1Leibniz Universität Hannover, Hannoversches Zentrum für Optische Technologien, Nienburger Straße 17, Hannover, D-30167, Germany. jonas.kanngiesser@hot.uni-hannover.de.
We developed a novel, compact system for enhanced optical coherence tomography (OCT) imaging. This method improves signal quality and imaging depth in scattering media like biological tissue.
Area of Science:
- Optical Imaging
- Biomedical Optics
- Metrology
Background:
- Spectral domain optical coherence tomography (SD-OCT) enables 3D imaging in scattering media.
- Structured illumination and beam shaping enhance SD-OCT performance, improving signal-to-noise ratio and penetration depth in biological tissues.
Purpose of the Study:
- To present a compact and easily implementable design for independent wavefront manipulation and beam shaping in interferometric OCT systems.
- To integrate this design into existing free-space SD-OCT systems by modifying only the source arm.
Main Methods:
- A single spatial light modulator is used for independent wavefront control in both reference and sample arms.
- Analytical and numerical analyses were performed to validate the design.
- Experimental data were collected to confirm theoretical predictions.
Main Results:
- The proposed system allows for independent wavefront manipulation and beam shaping.
- Experimental validation confirmed the theoretical analysis.
- The system demonstrated utility in wavefront sensorless adaptive optics and iterative optical wavefront shaping for OCT signal enhancement.
Conclusions:
- The developed system is highly versatile for applications requiring independent phase or wavefront control.
- It effectively enhances OCT signal in strongly scattering media.
- The design offers a practical solution for improving OCT imaging capabilities.
Related Concept Videos
Interference and Diffraction
Phase Contrast and Differential Interference Contrast Microscopy
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...

