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Ac154 carried out anti-apoptotic role during AcMNPV infection process in the host insect cells

Houlin Bai1, Aihua Liang1, Yuejun Fu2

  • 1Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Biotechnology, Shanxi University, Taiyuan, 030006, People's Republic of China.

Molecular and Cellular Biochemistry
|October 12, 2019
PubMed

Insights

AcMNPV

Area of Science:

  • * Virology
  • * Molecular Biology
  • * Cell Biology

Background:

  • * AcMNPV (Autographa californica multiple nucleopolyhedrovirus) serves as a model baculovirus.
  • * The function of the AcMNPV gene ac154 remains uncharacterized.
  • * Understanding Ac154's role is crucial for baculovirus research and applications.

Purpose of the Study:

  • * To investigate the function of the AcMNPV gene ac154 during viral infection in Sf9 cells.
  • * To determine Ac154's impact on viral replication and host cell processes.
  • * To explore Ac154's potential applications in protein expression systems and biopesticides.

Main Methods:

  • * Ac154 gene knockout and overexpression in Sf9 cells.
  • * Analysis of viral progeny (BV) production and yield.
  • * MTT assays to assess cell proliferation and apoptosis.
  • * Examination of anti-apoptotic (p35) and pro-apoptotic (SfP53) gene/protein expression.

Main Results:

  • * Ac154 localizes to both the nucleus and cytoplasm of infected Sf9 cells.
  • * Ac154 knockout reduced progeny virus yield but did not affect BV production.
  • * Ac154 promoted Sf9 cell proliferation and inhibited apoptosis.
  • * Ac154 modulated p35 transcription and SfP53 expression, delaying apoptosis.

Conclusions:

  • * Ac154 plays an auxiliary role in baculovirus replication by delaying host cell apoptosis.
  • * Regulation of p35 and SfP53 by Ac154 creates a favorable environment for progeny virus production.
  • * Ac154 has potential applications for improving baculovirus-based expression systems and biopesticides.
Keywords:
Ac154Autograph californica multicapsid nuclear polyhedrosis virus (AcMNPV)BiopesticideSpodoptera frugiperda 9 cell

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