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Ultrahigh-Density Screens for Genome-Wide Yeast EMAPs in a Single Plate
Katherine Licon1, John Paul Shen1, Brenton P Munson1,2
1Department of Medicine, UC San Diego, La Jolla, CA, USA.
Methods in Molecular Biology (Clifton, N.J.)
|October 12, 2019
Abstract:
Systematic measurements of genetic interactions have been used to classify gene functions and to categorize genes into protein complexes, functional pathways and biological processes. This protocol describes how to perform a high-throughput genetic interaction screen in S. cerevisiae using a variant of epistatic miniarray profiles (E-MAP) in which the fitnesses of 6144 colonies are measured simultaneously. We also describe the computational methods to analyze the resulting data.
