Protein-Protein Interaction Profiling in Candida albicans Revealed by Biochemical Purification-Mass Spectrometry
Reza Pourhaghighi1, Teresa R O'Meara2, Leah E Cowen2
1Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, ON, Canada.
Methods in Molecular Biology (Clifton, N.J.)
|October 12, 2019
Abstract:
BP/MS is a new experimental proteomic platform for systematic study of native protein complexes and global protein-protein interaction networks based on deep biochemical purification of soluble protein extracts and mass spectrometry identification of coeluting proteins. Herein, we describe the application of this methodology to draft a high-confidence protein interaction network for Candida albicans.
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