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Bioorthogonal Chemical Imaging of Cell Metabolism Regulated by Aromatic Amino Acids
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Activity-Based Sensing: A Synthetic Methods Approach for Selective Molecular Imaging and Beyond.

Kevin J Bruemmer1, Steven W M Crossley1, Christopher J Chang1,2

  • 1Department of Chemistry, University of California, Berkeley, Berkeley, CA, 94720, USA.

Angewandte Chemie (International Ed. in English)
|October 13, 2019
PubMed
Summary

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Activity-based sensing (ABS) uses chemical reactions, not just molecular recognition, to detect analytes. This versatile method is crucial for understanding biological signaling and stress pathways in complex environments.

Area of Science:

  • Supramolecular chemistry
  • Chemical biology
  • Chemical sensing

Background:

  • Activity-based sensing (ABS) evolved from supramolecular chemistry and selective chemical receptors.
  • ABS utilizes molecular reactivity for analyte detection, differing from traditional molecular recognition.
  • It investigates biological signaling and stress pathways, focusing on metal ions and small molecules.

Purpose of the Study:

  • To highlight the growth and distinctiveness of activity-based sensing as a research field.
  • To explain how chemical reactions are exploited for selective and sensitive detection in biological systems.
  • To showcase the broad applicability of ABS in various research and discovery areas.

Main Methods:

  • Leveraging the inherent chemical reactivity of biological species.
Keywords:
activity-based probesbio-orthogonal reactionsbioconjugation chemistrymolecular imagingsensors

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  • Developing selective and sensitive synthetic detection methods.
  • Applying reaction-driven approaches for in-situ analysis within complex living environments.

    • Main Results:

    • ABS enables the investigation of chemical species mediating biological signaling and stress pathways.
    • The approach is applicable across diverse imaging modalities, including fluorescence, luminescence, and PET.
    • ABS facilitates applications in drug discovery and materials science.

    Conclusions:

    • Activity-based sensing is a rapidly growing field with significant potential in biological and chemical research.
    • The reaction-driven nature of ABS offers a powerful tool for deciphering complex biological processes.
    • ABS methods are expanding into new frontiers like drug and materials discovery.