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Silencing TTK expression inhibits the proliferation and progression of prostate cancer

Saipeng Chen1, Jianan Wang1, Lin Wang1

  • 1Tianjin Institute of Urology, The Second Hospital of Tianjin Medical University, Tianjin, 300211, China.

Experimental Cell Research
|October 13, 2019
PubMed
Abstract

Insights

The study found that TTK gene expression correlates with prostate cancer malignancy. Inhibiting TTK in cells and animal models reduced tumor growth, suggesting TTK as a potential therapeutic target for prostate cancer.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Prostate cancer (PCa) progression is linked to changes in gene expression.
  • Identifying key genes driving PCa malignancy is crucial for developing new therapies.

Purpose of the Study:

  • To investigate differentially expressed genes in prostate cancer progression.
  • To functionally validate the role of selected genes, specifically TTK, in PCa.

Main Methods:

  • Transcriptome analysis of 499 PCa cases from TCGA database.
  • Selection and functional validation of TTK using siRNA and shRNA in PCa cell lines and in vivo models.
  • KEGG pathway enrichment analysis.

Main Results:

  • TTK expression positively correlates with Gleason score and is higher in metastatic PCa cell lines.
  • TTK knockout inhibited PCa cell proliferation, invasion, migration, and promoted apoptosis in vitro.
  • TTK knockout suppressed tumor formation in vivo and affected CDK2 and CCNE1 expression.

Conclusions:

  • TTK is significantly associated with prostate cancer malignancy.
  • TTK represents a potential novel therapeutic target for prostate cancer treatment.
Keywords:
Cell cycleGleason scoreProstate cancerTTK/Mps1

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