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Resolving electrical stimulus triggered molecular binding and force modulation upon thrombin-aptamer biointerface.

Xiao Ma1, Agnivo Gosai2, Pranav Shrotriya2

  • 1Department of Mechanical Engineering, Iowa State University, Ames, IA 50011, USA; Department of Biomedical Engineering, New York University, Brooklyn, NY 11201, USA.

Journal of Colloid and Interface Science
|October 13, 2019
PubMed
Summary
This summary is machine-generated.

Applying electrical fields can control the binding between thrombin and DNA aptamers. Positive potentials weaken binding, while negative potentials have no effect, enabling new biointerface applications.

Keywords:
Analytical modelingAptamerDynamic force spectroscopyElectrochemical atomic force microscopyElectrostatic actuationSingle energy barrier modelThrombin

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Area of Science:

  • Biophysics
  • Nanotechnology
  • Biochemistry

Background:

  • Thrombin is a key human coagulation protein.
  • DNA aptamers are highly specific binding agents.
  • Controlling biomolecular interactions is crucial for biosensing and drug delivery.

Purpose of the Study:

  • To investigate the effect of electrostatic fields on thrombin-DNA aptamer binding.
  • To determine the feasibility of using electrical potentials to modulate biomolecular interactions.
  • To explore potential applications in stimuli-responsive biointerfaces.

Main Methods:

  • Utilized atomic force microscopy (AFM) to measure binding forces.
  • Applied varying electrical potentials (-100, 0, 100 mV) to a gold substrate with a thiolated aptamer.
  • Employed computational analysis to model electrostatic interactions.

Main Results:

  • Positive electrode potential significantly reduced thrombin-aptamer binding strength and propensity.
  • Negative electrode potential showed no discernible effect on binding.
  • Theoretical analysis confirmed repulsive electrostatic forces under positive potential promote dissociation.

Conclusions:

  • Electrostatic fields can effectively modulate the binding interaction between thrombin and DNA aptamers.
  • This control offers potential for nanoscale manipulation of biointerfaces.
  • The findings support the development of stimuli-responsive bioelectronic devices.