Related Experiment Video
Updated: Jan 5, 2026
High-throughput Saccharification Assay for Lignocellulosic Materials
Published on: July 3, 2011
Improving enzymatic saccharification of hardwood through lignin modification by carbocation scavengers and the
Qiulu Chu1, Kai Song2, Jing Wang3
1School of Agricultural Equipment Engineering, Jiangsu University, Zhenjiang 212013, China.
Abstract:
In this work, the beneficial effect of carbocation scavenger additives on hardwood pretreatment was revealed by significantly improved biomass saccharification: cellulose hydrolysis yield was increased by over 15% after steam pretreatment of poplar, while that was enhanced by more than 48% after dilute acid pretreatment. Besides, the relative contributions of lignin towards enzyme binding and physical barrier effect for proposed mechanisms were quantified. Results indicated that the addition of carbocation scavenger, 2-naphthol-7-sulfonate, resulted in acid groups incorporation of 62.36 mmol/kg to lignin, which mitigated enzyme non-productive binding. Moreover, enlarged biomass porosity and reduced surface lignin coverage were detected through BET and XPS analysis, respectively, which mostly related to the diminished physical barrier effect of lignin. As a result, the lignin inhibitions were significantly suppressed through the addition of carbocation scavenger, giving rise to significantly improved enzymatic hydrolysis of hardwood.
