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Updated: Jan 5, 2026

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression
Published on: March 29, 2019
Utilizing intein trans-splicing for in vivo generation of site-specifically modified proteins
Igor Maksimovic1, Devin Ray2, Qingfei Zheng3
1Tri-Institutional PhD Program in Chemical Biology, New York, NY, United States; Chemical Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY, United States.
Abstract:
Many cellular processes as well as their associated pathologies are regulated by protein post-translational modifications (PTMs). Understanding the precise roles of these adducts hinges on the development of methods to robustly and site-specifically manipulate proteins in their physiological environments. Recently, ultrafast intein protein trans-splicing (PTS) was harnessed to incorporate site-specific modifications on cellular chromatin in live cells. In this chapter, we present the protocols for the generation of synthetic modifications on native chromatin as well as highlight the capabilities of this methodology.
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