Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Diabetic Foot Ulcer01:31

Diabetic Foot Ulcer

Definition A diabetic foot ulcer (DFU) is a chronic, non-healing wound that develops in individuals with diabetes. It typically occurs on pressure-bearing areas such as the heel, metatarsal heads, or hallux, and carries a high risk of infection and amputation.Pathophysiology • The development of DFUs can be explained by four interconnected mechanisms: neuropathy, ischemia, infection, and impaired wound healing. • Neuropathy is the most common factor. Sensory neuropathy reduces pain perception,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Protocol to Create Chronic Wounds in Diabetic Mice - an Update to the Published Protocol.

Journal of visualized experiments : JoVE·2025
Same author

The Impact of the Skin Microbiome and Oxidative Stress on the Initiation and Development of Cutaneous Chronic Wounds.

Antioxidants (Basel, Switzerland)·2025
Same author

Chronic Wound Initiation: Single-Cell RNAseq of Cutaneous Wound Tissue and Contributions of Oxidative Stress to Initiation of Chronicity.

Antioxidants (Basel, Switzerland)·2025
Same author

Integration of Functional Polymers and Biosensors to Enhance Wound Healing.

Advanced healthcare materials·2024
Same author

<i>Pseudomonas aeruginosa</i> Activates Quorum Sensing, Antioxidant Enzymes and Type VI Secretion in Response to Oxidative Stress to Initiate Biofilm Formation and Wound Chronicity.

Antioxidants (Basel, Switzerland)·2024
Same author

Assessing Animal Models to Study Impaired and Chronic Wounds.

International journal of molecular sciences·2024

Related Experiment Video

Updated: Jul 9, 2026

Come to the Light Side: In Vivo Monitoring of Pseudomonas aeruginosa Biofilm Infections in Chronic Wounds in a Diabetic Hairless Murine Model
09:15

Come to the Light Side: In Vivo Monitoring of Pseudomonas aeruginosa Biofilm Infections in Chronic Wounds in a Diabetic Hairless Murine Model

Published on: October 10, 2017

13.9K

Protocol to Create Chronic Wounds in Diabetic Mice.

Jane Hannah Kim1, Manuela Martins-Green2

  • 1Department of Molecular, Cell and Systems Biology, University of California, Riverside.

Journal of Visualized Experiments : Jove
|October 15, 2019
PubMed
Summary

Researchers developed a new mouse model for diabetic chronic wounds. This model mimics human chronic wounds, aiding research into healing defects and improving patient care.

More Related Videos

Protocol to Create Chronic Wounds in Diabetic Mice
06:55

Protocol to Create Chronic Wounds in Diabetic Mice

Published on: September 25, 2019

21.5K
A Protocol for Constructing a Rat Wound Model of Type 1 Diabetes
05:18

A Protocol for Constructing a Rat Wound Model of Type 1 Diabetes

Published on: February 17, 2023

5.5K

Related Experiment Videos

Last Updated: Jul 9, 2026

Come to the Light Side: In Vivo Monitoring of Pseudomonas aeruginosa Biofilm Infections in Chronic Wounds in a Diabetic Hairless Murine Model
09:15

Come to the Light Side: In Vivo Monitoring of Pseudomonas aeruginosa Biofilm Infections in Chronic Wounds in a Diabetic Hairless Murine Model

Published on: October 10, 2017

13.9K
Protocol to Create Chronic Wounds in Diabetic Mice
06:55

Protocol to Create Chronic Wounds in Diabetic Mice

Published on: September 25, 2019

21.5K
A Protocol for Constructing a Rat Wound Model of Type 1 Diabetes
05:18

A Protocol for Constructing a Rat Wound Model of Type 1 Diabetes

Published on: February 17, 2023

5.5K

Area of Science:

  • Biomedical Science
  • Wound Healing Research
  • Animal Models

Background:

  • Chronic wounds affect millions, incurring significant healthcare costs.
  • Defective regulation of cellular and molecular processes underlies chronic wound development.
  • Existing research has not fully elucidated the mechanisms of chronic wound formation.

Purpose of the Study:

  • To introduce a novel mouse model for studying diabetic chronic wounds.
  • To provide a reproducible method for generating chronic wounds in a preclinical setting.
  • To facilitate a deeper understanding of the pathophysiology of chronic wound development.

Main Methods:

  • Utilized db/db-/- mice to create a diabetic chronic wound model.
  • Induced high levels of oxidative stress (OS) post-wounding using specific antioxidant enzyme inhibitors (catalase and glutathione peroxidase).
  • Characterized the resulting wounds for OS levels, biofilm formation, and chronicity duration.

Main Results:

  • The developed model exhibits key characteristics of human diabetic chronic wounds.
  • Wounds demonstrated elevated OS, natural biofilm development, and became chronic within 20 days.
  • Wounds remained open for over 60 days, indicating a persistent chronic state.

Conclusions:

  • This novel mouse model offers a significant advancement for chronic wound research.
  • The model's fidelity to human chronic wounds can accelerate the understanding of healing defects.
  • Findings from this model hold potential to improve therapeutic strategies and patient outcomes for chronic wounds.