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Genomic epidemiology of penicillin-non-susceptible Streptococcus pneumoniae

Tamsin C M Dewé1, Joshua C D'Aeth1, Nicholas J Croucher1

  • 1MRC Centre for Global Infectious Disease Analysis, Department of Infectious Disease Epidemiology, St. Mary's Campus, Imperial College London, London, W2 1PG, UK.

Microbial Genomics
|October 15, 2019
PubMed

Insights

Penicillin-non-susceptible Streptococcus pneumoniae (PNSP) strains have evolved mosaic genes through recombination, leading to widespread resistance. Understanding PNSP genetics aids in tracking their evolution and improving detection methods.

Area of Science:

  • Microbiology
  • Genetics
  • Evolutionary Biology

Background:

  • Penicillin-non-susceptible Streptococcus pneumoniae (PNSP) emerged in the 1960s and are now globally prevalent.
  • PNSP strains are characterized by mosaic pbp2x, pbp2b, and pbp1a genes resulting from interspecies recombination.

Purpose of the Study:

  • To investigate the genetic basis of PNSP non-susceptibility.
  • To enhance understanding of PNSP evolution and improve bioinformatic detection methods.

Main Methods:

  • Molecular microbiology techniques.
  • Genome-wide association studies (GWAS).
  • Epistasis analyses.

Main Results:

  • The extent of mosaic gene alterations correlates with the range and concentration of beta-lactam resistance.
  • Genetic complexity underlies PNSP phenotypes.

Conclusions:

  • Understanding PNSP genetic evolution is crucial for effective genomic surveillance.
  • Improved bioinformatic tools can enhance the identification of PNSP from genomic data.
Keywords:
antibiotic resistancegenomic epidemiologypenicillinpneumococcus

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