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Quantitative Analysis of Alternative Pre-mRNA Splicing in Mouse Brain Sections Using RNA In Situ Hybridization Assay
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Quantitative Analysis of Alternative Pre-mRNA Splicing in Mouse Brain Sections Using RNA In Situ Hybridization Assay

Published on: August 26, 2018

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Identification of neoantigens derived from alternative splicing and RNA modification

Jiyeon Park1,2, Yeun-Jun Chung1,2,3

  • 1Precision Medicine Research Center, College of Medicine, The Catholic University of Korea, Seoul 06591, Korea.

Genomics & Informatics
|October 15, 2019
PubMed

Insights

Cancer immunotherapy can target neoantigens from mutated genes. This review explores alternative splicing and RNA editing as sources of novel neoantigens and discusses methods for their identification using RNA sequencing.

Area of Science:

  • Oncology
  • Immunology
  • Genomics

Background:

  • Somatic mutations drive cancer development.
  • Tumor-specific neoantigens, derived from mutated genes, are recognized by the immune system.
  • Neoantigen-based immunotherapy holds promise for personalized cancer treatment but faces discovery challenges.

Purpose of the Study:

  • To review recent advancements in neoantigen discovery.
  • To highlight alternative splicing and RNA editing as key mechanisms of neoantigen generation.
  • To describe strategies for predicting and validating neoantigens from RNA sequencing data.

Main Methods:

  • Large-scale screening of neoantigens generated by alternative splicing.
  • Analysis of neoantigens produced through RNA editing.
  • Utilizing RNA sequencing data for neoantigen prediction and validation.

Main Results:

  • Alternative splicing and RNA editing are significant contributors to neoantigen diversity.
  • RNA sequencing provides a powerful tool for identifying novel neoantigens.
  • Established strategies facilitate the prediction and validation of neoantigens.

Conclusions:

  • Understanding neoantigen generation mechanisms is crucial for advancing cancer immunotherapy.
  • Alternative splicing and RNA editing represent promising avenues for discovering new therapeutic targets.
  • Improved strategies for neoantigen identification are essential for realizing the full potential of personalized neoantigen-based therapies.
Keywords:
RNA editingalternative splicingneoantigen

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