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Adherence of Bacteria to Plant Surfaces Measured in the Laboratory
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Bacterial survival in microscopic surface wetness.

Maor Grinberg1, Tomer Orevi1, Shifra Steinberg1

  • 1Department of Plant Pathology and Microbiology, Robert H. Smith Faculty of Agriculture, Food, and Environment, Hebrew University, Rehovot, Israel.

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|October 16, 2019
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Summary

Microscopic water droplets form around bacterial aggregates on plant leaves, aiding survival. Larger droplets, formed by hygroscopic aerosols, significantly enhance bacterial survival during dry daytime conditions.

Keywords:
Pseudomonas fluorescensPseudomonas putidacomputational biologydeliquescencedesiccationinfectious diseasemicrobiologyphyllosphereplant microbiomesystems biology

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Area of Science:

  • Microbiology
  • Plant Science
  • Environmental Science

Background:

  • Plant leaves host a vast microbial community crucial for global ecosystems.
  • Mechanisms of microbial survival against desiccation on leaf surfaces during daytime are poorly understood.
  • Microscopic surface wetness, persisting via deliquescence of hygroscopic aerosols, may play a role.

Purpose of the Study:

  • To investigate the role of microscopic surface wetness in microbial cell survival on drying surfaces.
  • To understand how bacterial aggregates interact with and utilize microdroplets for survival.

Main Methods:

  • Studied bacterial survival on drying surfaces under moderate humidity.
  • Observed microdroplet formation around bacterial aggregates using microscopy.
  • Quantified the relationship between aggregate size, droplet size, and cell survival.
  • Tested survival across 13 bacterial species, including in-depth analysis of *Pseudomonas fluorescens* and *P. putida*.

Main Results:

  • Stable microdroplets form around bacterial aggregates on drying surfaces due to capillary pinning and deliquescence.
  • Microdroplet size increases with bacterial aggregate size.
  • Bacterial cell survival is significantly higher in the presence of larger microdroplets.
  • This phenomenon is consistent across multiple bacterial species.

Conclusions:

  • Microdroplet formation around bacterial aggregates is a key survival mechanism for microbes on plant leaves.
  • This process is vital for bacterial survival in unsaturated environments, such as leaf surfaces.
  • The findings offer insights into microbial resilience in terrestrial ecosystems.