Endogenous intronic antisense long non-coding RNA, MGAT3-AS1, and kidney transplantation
Subagini Nagarajah1,2, Shengqiang Xia3, Marianne Rasmussen1
1Odense University Hospital, Department of Nephrology, Odense, Denmark.
Scientific Reports
|October 16, 2019
Summary
Low levels of the long non-coding RNA MGAT3-AS1 before kidney transplantation predict delayed graft function. This finding offers a potential biomarker for assessing short-term outcomes in deceased donor kidney transplant recipients.
Area of Science:
- Immunology
- Molecular Biology
- Transplantation Science
Background:
- The β-1,4-mannosylglycoprotein 4-β-N-acetylglucosaminyltransferase (MGAT3) enzyme is crucial for innate immunity.
- Long non-coding RNAs (lncRNAs) are increasingly recognized for their roles in biological processes and disease.
- MGAT3-AS1, an intronic antisense lncRNA, has not been extensively studied in the context of organ transplantation.
Purpose of the Study:
- To investigate the potential of MGAT3-AS1 as a predictive biomarker for delayed allograft function after kidney transplantation.
- To assess the association between MGAT3-AS1 levels and kidney transplant outcomes.
- To evaluate the diagnostic accuracy of MGAT3-AS1 in predicting delayed graft function.
Main Methods:
- A prospective study involving 129 deceased donor kidney transplant recipients.
- Measurement of MGAT3-AS1 levels in mononuclear cells using quantitative reverse transcription PCR (qRT-PCR) pre- and post-transplantation.
- Definition of delayed graft function as requiring at least one dialysis session within 7 days post-transplantation.
Main Results:
- Delayed graft function occurred in 17% (22/129) of recipients.
- Significantly lower median MGAT3-AS1 levels were observed post-transplantation in patients with delayed graft function compared to those with immediate graft function (p < 0.05).
- Preoperative MGAT3-AS1 levels were significantly lower in recipients who developed delayed graft function (p < 0.05), with an area under the receiver-operator characteristic curve of 0.83 (p < 0.01), indicating predictive value.
Conclusions:
- Preoperative MGAT3-AS1 levels can predict delayed graft function in deceased donor kidney transplant recipients.
- MGAT3-AS1 serves as a potential non-invasive biomarker for assessing short-term kidney allograft outcomes.
- Further research may explore the functional role of MGAT3-AS1 in transplant immunology.
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