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Molecular isomerization triggered by H2S to an NIR accessible first direct visualization of Ca2+-dependent production
Ying Wen1, Fangjun Huo2, Junping Wang1
1Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Key Laboratory of Materials for Energy Conversion and Storage of Shanxi Province, Institute of Molecular Science, Shanxi University, Taiyuan 030006, China. yincx@sxu.edu.cn.
Abstract:
Few studies determined the role of intracellular labile Ca2+ in H2S homeostasis. Undoubtedly, fluorescent probes are powerful tools for exploring the question because of their unique advantages: non-destruction, visualization, and multi-levels imaging. Herein, a near-infrared (λem = 687 nm) and methylene blue chromophore-based fluorescent probe (MB1) for H2S was rationally developed. Based on its high sensitivity and selectivity, MB1 was employed to image the concentration change of H2S, upon stimulating it with ionomycin (a specific calcium ionophore). We found that the intracellular labile Ca2+ acted as a promotor for H2S production in living cells. Furthermore, cystathionine γ-lyase (CSE) might have functioned as a positive mediator of Ca2+-dependent H2S production. These direct and visible links for H2S/Ca2+ will help us to understand the complex signaling in a better way.
