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Reduced Expression of Chl1 gene Impairs Insulin Secretion by Down-Regulating the Expression of Key Molecules of

Jalal Taneera1, Sarah Dhaiban1, Mahmood Hachim1

  • 1Sharjah Institute for Medical Research, University of Sharjah, Sharjah, United Arab Emirates.

Experimental and Clinical Endocrinology & Diabetes : Official Journal, German Society of Endocrinology [And] German Diabetes Association
|October 16, 2019
PubMed

Insights

Silencing the Chl1 gene impairs pancreatic beta-cell function by reducing insulin secretion and content. This disruption affects key molecules involved in insulin biosynthesis and signaling pathways.

Area of Science:

  • Endocrinology
  • Molecular Biology
  • Genetics

Background:

  • Chl1 gene expression silencing reduces insulin secretion, but the mechanism is unclear.
  • CHL1 is highly expressed in pancreatic islets and adipose tissues.
  • Diabetic islets show lower CHL1 expression compared to non-diabetic islets.

Purpose of the Study:

  • Investigate the mechanism by which Chl1 affects insulin secretion in INS-1 cells.
  • Assess the impact of Chl1 silencing on insulin secretion, content, cell viability, and apoptosis.
  • Identify molecular signatures associated with Chl1 expression silencing using gene set enrichment analysis (GSEA).

Main Methods:

  • RNA-sequencing to analyze CHL1 expression in human tissues and INS-1 cells.
  • Silencing of Chl1 in INS-1 cells.
  • Measurement of insulin secretion and content.
  • Assessment of cell viability, apoptosis, and proliferation.
  • Gene set enrichment analysis (GSEA).

Main Results:

  • CHL1 expression is high in islets and adipose tissue, inversely correlated with HbA1c and BMI.
  • Silencing Chl1 in INS-1 cells significantly reduced insulin content and secretion.
  • Key beta-cell function molecules (Insulin, Pdx1, Gck, Glut2, Insrβ) were downregulated.
  • Cell viability, apoptosis, and proliferation remained unaffected.
  • GSEA indicated that the insulin-signaling pathway was influenced by Chl1 silencing.

Conclusions:

  • Chl1 silencing impairs pancreatic beta-cell function by disrupting insulin biosynthesis and secretion.
  • The study identifies Chl1 as a crucial factor in maintaining beta-cell function.
  • Chl1 plays a significant role in regulating insulin signaling pathways.

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