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FRET Microscopy in Yeast.

Michal Skruzny^1,2, Emma Pohl^3,4, Marc Abella^3,4

¹Department of Systems and Synthetic Microbiology, Max Planck Institute for Terrestrial Microbiology, 35043 Marburg, Germany. michal.skruzny@synmikro.mpi-marburg.mpg.de.

|October 17, 2019

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Förster resonance energy transfer (FRET) microscopy visualizes nanoscale protein organization in living cells. This guide details FRET techniques and biosensors for yeast models, aiding research in cell biology and drug discovery.

Area of Science:

Biophysics
Cell Biology
Microscopy

Background:

Förster resonance energy transfer (FRET) microscopy is crucial for studying nanoscale organization of multiprotein assemblies in vivo.
FRET biosensors enable real-time monitoring of biochemical and biophysical processes within living cells.

Purpose of the Study:

To summarize existing FRET experiments and biosensors in yeast models (Saccharomyces cerevisiae and Schizosaccharomyces pombe).
To provide a practical guide for successful FRET experiments in yeast, covering techniques, fluorescent proteins, and experimental setups.

Main Methods:

Review of existing literature on FRET applications in yeast.
Compilation of suitable FRET techniques, fluorescent proteins, and experimental setups.

Keywords:

FLIM GFP acceptor photobleaching budding yeast fission yeast ratiometric FRET sensitized emission

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Main Results:

Identification and summary of current FRET experiments and biosensors applicable to yeast.
Guidance on selecting appropriate FRET tools and methodologies for yeast research.

Conclusions:

Yeast models are valuable platforms for FRET studies in fundamental biomedical research.
This guide facilitates the application of advanced FRET microscopy in yeast for understanding cellular processes.