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Updated: Jan 5, 2026

Imaging and Analysis for Quantifying Maize (Zea mays) Abiotic Stress Phenotypes
Published on: March 28, 2025
Capturing Maize Stand Heterogeneity Across Yield-Stability Zones Using Unmanned Aerial Vehicles (UAV)
Guanyuan Shuai1, Rafael A Martinez-Feria2, Jinshui Zhang3
1Department of Earth and Environmental Sciences, Michigan State University, East Lansing, MI 48823, USA. shuaigua@msu.edu.
Abstract:
Despite the new equipment capabilities, uneven crop stands are still common occurrences in crop fields, mainly due to spatial heterogeneity in soil conditions, seedling mortality due to herbivore predation and disease, or human error. Non-uniform plant stands may reduce grain yield in crops like maize. Thus, detecting signs of variability in crop stand density early in the season provides critical information for management decisions and crop yield forecasts. Processing techniques applied on images captured by unmanned aerial vehicles (UAVs) has been used successfully to identify crop rows and estimate stand density and, most recently, to estimate plant-to-plant interval distance. Here, we further test and apply an image processing algorithm on UAV images collected from yield-stability zones in a commercial crop field. Our objective was to implement the algorithm to compare variation of plant-spacing intervals to test whether yield differences within these zones are related to differences in crop stand characteristics. Our analysis indicates that the algorithm can be reliably used to estimate plant counts (precision >95% and recall >97%) and plant distance interval (R ~0.9 and relative error <10%). Analysis of the collected data indicated that plant spacing variability differences were small among plots with large yield differences, suggesting that it was not a major cause of yield variability across zones with distinct yield history. This analysis provides an example of how plant-detection algorithms can be applied to improve the understanding of patterns of spatial and temporal yield variability.
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