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Turbidimetry on Human Washed Platelets: The Effect of the Pannexin1-inhibitor Brilliant Blue FCF on Collagen-induced Aggregation
Published on: April 6, 2017
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Smoking cessation affects human platelet activation induced by collagen
Yuko Kito1,2, Mami Iida3, Kumiko Tanabe1
1Department of Anesthesiology and Pain Medicine, Gifu University Graduate School of Medicine, Gifu 501-1194, Japan.
Experimental and Therapeutic Medicine
|October 17, 2019
Summary
Smoking cessation temporarily increases human platelet activation, posing short-term risks. Platelet aggregation and growth factor secretion rise post-cessation, returning to baseline after 12 weeks. This highlights the importance of considering abstinence duration.
Area of Science:
- Cardiovascular Science
- Hemostasis and Thrombosis
- Public Health
Background:
- Smoking is a known risk factor for cardiovascular diseases.
- The impact of smoking on human platelet activation is debated.
- Smoking cessation is recommended before surgery due to increased risks.
Purpose of the Study:
- To investigate the effects of smoking cessation on human platelet activation.
- To assess changes in platelet aggregation and signaling pathways after smoking cessation.
Main Methods:
- Studied 19 patients undergoing smoking cessation.
- Collected blood samples at baseline, 4, 8, and 12 weeks post-cessation.
- Measured platelet aggregation and collagen-induced PDGF-AB secretion using laser scattering and assessed p38 MAP kinase phosphorylation.
Main Results:
- Collagen-induced platelet aggregation increased at 4 and 8 weeks post-cessation.
- Platelet-derived growth factor-AB secretion was elevated at 4 and 8 weeks.
- Collagen-induced p38 MAP kinase phosphorylation was enhanced after 4 weeks.
- Platelet activity returned to baseline levels by 12 weeks.
Conclusions:
- Smoking cessation leads to temporary, short-term human platelet hyper-activation.
- This hyper-reactivity may increase the risk of thrombotic complications.
- Considering the duration of smoking abstinence is crucial for managing patient risk.
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