Multifluorescence Live Analysis of Herpes Simplex Virus Type-1 Replication
Michael Seyffert1, Cornel Fraefel2
1Institute of Virology, University of Zurich, Zürich, Switzerland. michael.seyffert@uzh.ch.
Abstract:
The possibility to label specific viral and cellular structures with live cell markers such as autofluorescent proteins has greatly contributed to our understanding of diverse steps of the virus life cycle, as it allows for monitoring virus replication in a spatial and temporal fashion. Here, we describe the multifluorescent live analysis of the multicompartment Herpes Simplex Virus Type-1 (HSV-1) by live cell confocal laser scanning microscopy.
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