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Related Experiment Video

Updated: Jan 5, 2026

Temporal Analysis of the Nuclear-to-cytoplasmic Translocation of a Herpes Simplex Virus 1 Protein by Immunofluorescent Confocal Microscopy

Temporal Analysis of the Nuclear-to-cytoplasmic Translocation of a Herpes Simplex Virus 1 Protein by Immunofluorescent Confocal Microscopy

Published on: November 4, 2018

Multifluorescence Live Analysis of Herpes Simplex Virus Type-1 Replication.

Michael Seyffert¹, Cornel Fraefel²

¹Institute of Virology, University of Zurich, Zürich, Switzerland. michael.seyffert@uzh.ch.

Methods in Molecular Biology (Clifton, N.J.)

|October 17, 2019

Summary

Live cell markers and confocal microscopy enable detailed observation of Herpes Simplex Virus Type-1 (HSV-1) replication. This study visualizes viral and cellular structures during the virus life cycle.

Keywords:

Confocal laser scanning microscopy Image processing Live cell imaging Multifluorescent recombinant HSV-1

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Published on: October 29, 2020

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Last Updated: Jan 5, 2026

Temporal Analysis of the Nuclear-to-cytoplasmic Translocation of a Herpes Simplex Virus 1 Protein by Immunofluorescent Confocal Microscopy

Temporal Analysis of the Nuclear-to-cytoplasmic Translocation of a Herpes Simplex Virus 1 Protein by Immunofluorescent Confocal Microscopy

Published on: November 4, 2018

Live Cell Imaging of Alphaherpes Virus Anterograde Transport and Spread

Live Cell Imaging of Alphaherpes Virus Anterograde Transport and Spread

Published on: August 16, 2013

Single-Cell Multiplexed Fluorescence Imaging to Visualize Viral Nucleic Acids and Proteins and Monitor HIV, HTLV, HBV, HCV, Zika Virus, and Influenza Infection

Single-Cell Multiplexed Fluorescence Imaging to Visualize Viral Nucleic Acids and Proteins and Monitor HIV, HTLV, HBV, HCV, Zika Virus, and Influenza Infection

Published on: October 29, 2020

Area of Science:

Virology
Cell Biology
Microscopy

Background:

Live cell markers like autofluorescent proteins enhance understanding of viral life cycles.
Monitoring virus replication requires spatial and temporal analysis.

Purpose of the Study:

To describe the multifluorescent live analysis of Herpes Simplex Virus Type-1 (HSV-1).
To utilize live cell confocal laser scanning microscopy for studying HSV-1.

Main Methods:

Employing multifluorescent live analysis techniques.
Utilizing live cell confocal laser scanning microscopy.
Labeling specific viral and cellular structures.

Main Results:

Detailed visualization of HSV-1 replication dynamics.
Spatial and temporal monitoring of virus-host interactions.
Characterization of multicompartment aspects of HSV-1.

Conclusions:

Multifluorescent live analysis provides powerful insights into HSV-1 biology.
Confocal microscopy is effective for studying virus replication in real-time.
This approach advances the understanding of the HSV-1 life cycle.