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Published on: September 3, 2012
The Murine Intravaginal HSV-2 Challenge Model for Investigation of DNA Vaccines
Joshua O Marshak1, Lichun Dong1, David M Koelle2,3,4,5,6
1Department of Medicine, University of Washington, Seattle, WA, USA.
Abstract:
DNA vaccines have been licensed in veterinary medicine and have promise for humans. This format is relatively immunogenic in mice and guinea pigs, the two principle HSV-2 animal models, permitting rapid assessment of vectors, antigens, adjuvants, and delivery systems. Limitations include the relatively poor immunogenicity of naked DNA in humans and the profound differences in HSV-2 pathogenesis between host species. Herein, we detail lessons learned investigating candidate DNA vaccines in the progesterone-primed female mouse vaginal model of HSV-2 infection as a guide to investigators in the field.
