Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Chromosome Replication02:31

Chromosome Replication

10.4K
Before a cell can divide, it must accurately replicate all of its chromosomes, including the DNA and its associated histone and non-histone proteins.  This process begins at numerous origins of replication during the S phase of the cell cycle in each of a cell’s chromosomes simultaneously. Certain nucleotides can act as origins of replication, but these sequences are not well defined - especially in complex, multi-cellular, eukaryotic species. The length of DNA that spans an origin...
10.4K
Chromosome Structure02:40

Chromosome Structure

25.7K
A functional eukaryotic chromosome must contain three elements: a centromere, telomeres, and numerous origins of replication.
The centromere is a DNA sequence that links sister chromatids. This is also where kinetochores, protein complexes to which spindle microtubules attach, are constructed after the chromosome is replicated. The kinetochores allow the spindle microtubules to move the chromosomes within the cell during cell division.
Telomeres consist of non-coding repetitive nucleotide...
25.7K
Replication in Eukaryotes01:29

Replication in Eukaryotes

16.9K
In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
16.9K
Replication in Eukaryotes02:31

Replication in Eukaryotes

202.3K
Overview
202.3K
The DNA Replication Fork01:02

The DNA Replication Fork

40.2K
An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication...
40.2K
The DNA Replication Fork01:02

The DNA Replication Fork

18.0K
18.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The subpleural pulmonary microvasculature in newborn yak (Bos grunniens).

Veterinary research communications·2008
Same author

Experimental confirmation of potential swept source optical coherence tomography performance limitations.

Applied optics·2008
Same author

A germin-like protein gene family functions as a complex quantitative trait locus conferring broad-spectrum disease resistance in rice.

Plant physiology·2008
Same author

[Spatial and temporal changes of palatal cell proliferation and cell apoptosis of retinoic acid induced mouse cleft palate in different embryonic stages].

Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology·2008
Same author

Identification of an Atlantic salmon IFN multigene cluster encoding three IFN subtypes with very different expression properties.

Developmental and comparative immunology·2008
Same author

Non-Gaussian statistics and superdiffusion in a driven-dissipative dusty plasma.

Physical review. E, Statistical, nonlinear, and soft matter physics·2008

Related Experiment Video

Updated: Jan 5, 2026

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
06:40

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

Published on: March 22, 2018

6.2K

iRO-PsekGCC: Identify DNA Replication Origins Based on Pseudo k-Tuple GC Composition.

Bin Liu1,2, Shengyu Chen3, Ke Yan4

  • 1School of Computer Science and Technology, Beijing Institute of Technology, Beijing, China.

Frontiers in Genetics
|October 18, 2019
PubMed
Summary

Identifying DNA replication origins is crucial for understanding DNA replication mechanisms. The new iRO-PsekGCC predictor, using Pseudo k-tuple GC Composition, improves accuracy in identifying these origins in yeast species.

Keywords:
DNA sequence analysispseudo k-tuple GC compositionrandom forestreplication origin identificationweb-server

More Related Videos

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
08:53

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Published on: May 2, 2025

849
Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement
08:06

Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement

Published on: January 19, 2017

8.8K

Related Experiment Videos

Last Updated: Jan 5, 2026

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
06:40

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

Published on: March 22, 2018

6.2K
Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
08:53

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Published on: May 2, 2025

849
Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement
08:06

Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement

Published on: January 19, 2017

8.8K

Area of Science:

  • Genomics
  • Molecular Biology
  • Bioinformatics

Background:

  • Identifying DNA replication origins is vital for comprehending DNA replication mechanisms and sequence analysis.
  • Existing computational methods, like iRO-3wPseKNC, have advanced origin identification but can be further improved.

Purpose of the Study:

  • To develop a more accurate computational tool for identifying DNA replication origins.
  • To capture the GC asymmetry bias in yeast species using an enhanced sequence composition approach.

Main Methods:

  • Proposed the Pseudo k-tuple GC Composition (PsekGCC) approach, incorporating GC skew and k-tuple GC Composition (k-GCC) effects.
  • Developed a new predictor, iRO-PsekGCC, based on the PsekGCC method.
  • Validated the predictor using rigorous jackknife tests on benchmark datasets from *Saccharomyces cerevisiae* and *Pichia pastoris*.

Main Results:

  • The iRO-PsekGCC predictor demonstrated superior performance compared to the existing iRO-3wPseKNC predictor.
  • The PsekGCC approach effectively captured the GC asymmetry bias in yeast DNA sequences.
  • The predictor achieved high accuracy in identifying DNA replication origins in the tested yeast species.

Conclusions:

  • iRO-PsekGCC represents a significant advancement in computational tools for DNA replication origin identification.
  • The PsekGCC method offers a robust way to analyze sequence composition for biological pattern recognition.
  • This tool is expected to be valuable for researchers in genomics and molecular biology studying DNA replication.