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Published on: June 24, 2008
Extraction and Characterization of Collagen with Cost-Effective Method from Human Placenta for Biomedical
Ali Karami1,2, Hamid Tebyanian2, Reza Sayyad Soufdoost3
1Nanobiotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Background:
Collagen is the main product in pharmaceutics and food industry with a high demand. Collagen can be extracted from several tissues such as skin, bone and tendon, etc. Collagen can be used in tissue engineering researches as a substrate of wound healing and nerve regeneration. Extraction methods of collagen are various with different purities. In this research, we aimed to extract collagen from human placenta with a modified method.
Methods:
This modified approach was used for extracting of collagen from human placenta with acetic acid and NaCl treatment using different concentrations.
Results:
SDS page showed three different bands that reflected two alpha-chains and one beta-chain with molecular weights of 102, 118 and 220 kDa, respectively. There was no significant difference between extracted collagen from human placenta and standard collagen in western blot analysis.
Conclusion:
It was concluded that human placenta can be an alternative source of collagen with high purity for biomedical applications such as tissue engineering, stem cell therapy and research.
