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LncRNA WT1-AS Inhibits Triple-Negative Breast Cancer Cell Migration and Invasion by Downregulating Transforming

Jiangfen Wang1, Chunfang Xi1, Xuan Yang1

  • 1Department of General Surgery, Shanxi Provincial People's Hospital, Taiyuan City, P.R. China.

Cancer Biotherapy & Radiopharmaceuticals
|October 18, 2019
PubMed

Insights

Long noncoding RNA WT1-AS acts as a tumor suppressor in triple-negative breast cancer (TNBC). It downregulates transforming growth factor β1 (TGF-β1), inhibiting TNBC cell migration and invasion.

Area of Science:

  • Molecular Biology
  • Oncology
  • Genetics

Background:

  • Long noncoding RNA WT1-AS is a potential tumor suppressor in gastric cancer.
  • The role of WT1-AS in other cancers, including triple-negative breast cancer (TNBC), is not well understood.

Purpose of the Study:

  • To investigate the function of WT1-AS in triple-negative breast cancer (TNBC).
  • To explore the relationship between WT1-AS and transforming growth factor β1 (TGF-β1) in TNBC.

Main Methods:

  • Gene expression analysis (qPCR, Western blot) in 62 TNBC patient tissues and BT-549 cell line.
  • Overexpression experiments to analyze gene interactions and regulatory effects.
  • Transwell assays to assess the impact of WT1-AS and TGF-β1 on cell invasion and migration.

Main Results:

  • WT1-AS expression was downregulated in TNBC tissues and correlated with advanced clinical stages.
  • Transforming growth factor β1 (TGF-β1) was upregulated in TNBC tissues and inversely correlated with WT1-AS.
  • WT1-AS overexpression inhibited TNBC cell migration and invasion, while TGF-β1 overexpression reversed these effects.
  • WT1-AS was found to negatively regulate TGF-β1 expression.

Conclusions:

  • WT1-AS functions as a tumor suppressor in TNBC by downregulating TGF-β1.
  • Inhibition of TNBC cell migration and invasion by WT1-AS is mediated through the TGF-β1 pathway.
Keywords:
TGF-β1WT1-ASinvasionmigrationtriple-negative breast cancer

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