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Aggregation induced emission-active two-photon absorption zwitterionic chromophore for bioimaging application.

Fei Meng1, Chengkai Zhang2, Dandan Li2

  • 1Institute of Physics Science and Information Technology, College of Chemistry and Chemical Engineering, Key Laboratory of Functional Inorganic Materials Chemistry of Anhui Province, Anhui Province Key Laboratory of Chemistry for Inorganic/Organic Hybrid Functionalized Materials, Key Laboratory of Structure and Functional Regulation of Hybrid Materials of Ministry of Education, Anhui University, Hefei, 230601, PR China; Department of Food and Environmental Engineering, Chuzhou Vocational and Technical College, Chuzhou, 239000, PR China.

Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
|October 18, 2019
PubMed
Summary

Researchers developed a novel zwitterionic chromophore for advanced bioimaging. This material enables high-resolution imaging with reduced phototoxicity, targeting cellular mitochondria.

Keywords:
Aggregation induced emissionBioimagingMitochondriaTwo-photon

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Area of Science:

  • Materials Science
  • Biomedical Imaging
  • Organic Chemistry

Background:

  • High-resolution bioimaging is crucial for understanding cellular processes.
  • Two-photon absorption materials offer advantages like low phototoxicity and deep tissue penetration.
  • Developing sophisticated fluorescent probes remains a key challenge in bioimaging.

Purpose of the Study:

  • To design and synthesize a novel zwitterionic chromophore with two-photon absorption properties.
  • To evaluate the fluorescent characteristics of the synthesized material in aggregated states.
  • To explore the potential of the material as a two-photon fluorescent probe for targeted bioimaging.

Main Methods:

  • Rational design and synthesis of a zwitterionic chromophore (MF) with a Donor-π-Acceptor (D-π-A) configuration.
  • Encapsulation of the chromophore into nanoparticles (MFNPs) using Pluronic F-127.
  • Characterization of one- and two-photon fluorescence properties.
  • Bioimaging studies using two-photon confocal microscopy and stimulated emission depletion nanoscopy.

Main Results:

  • The synthesized zwitterionic chromophore (MF) demonstrated enhanced one- and two-photon fluorescence in aggregated states.
  • MFNPs were successfully prepared and showed potential as two-photon fluorescent probes.
  • MFNPs exhibited specific targeting of mitochondria within cells.

Conclusions:

  • The novel zwitterionic chromophore MF is a promising material for advanced bioimaging applications.
  • MFNPs can serve as effective two-photon fluorescent probes for mitochondria visualization.
  • The developed material facilitates high-resolution bioimaging with reduced phototoxicity.