Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Beyond Berlin: The multidimensional evolution of ARDS in the era of precision critical care.

Journal of intensive medicine·2026
Same author

Novel Phenotypes of Acute Respiratory Failure and Differential Response to Awake Prone Positioning: A Multi-Cohort Study.

MedComm·2026
Same author

Video-Based Training on Physicians' Proficiency in Invasive Fungal Disease: A Multicentre, Cluster Randomised Controlled Trial.

Mycoses·2026
Same author

Design, Synthesis, and Structure-Activity Optimization of Marine-Inspired Macrolactones as Nanomolar Anticancer Agents.

ACS medicinal chemistry letters·2026
Same author

The janus-faced extracellular matrix remodeling in acute lung injury: a gatekeeper of macrophage localization and phenotype.

Science China. Life sciences·2026
Same author

Comparison of laparoscopic versus open approach surgery for gastric gastrointestinal stromal tumors in unfavorable surgical locations.

BMC cancer·2026
Same journal

The mitochondrial protease, LonP1, is a potential cardioprotective target for attenuating doxorubicin-induced cardiomyocyte death.

Journal of translational medicine·2026
Same journal

Enhancing the positive predictive value of early-stage ovarian cancer detection using a two-step machine learning framework.

Journal of translational medicine·2026
Same journal

CTSS regulates macrophage lipid metabolic reprogramming and white matter repair after intracerebral hemorrhage.

Journal of translational medicine·2026
Same journal

High glucose promotes aggressive progression of PR-negative endometrial cancer via CD146.

Journal of translational medicine·2026
Same journal

Multiphasic blood transcriptomic signatures of radioprotection by BIO 300, a synthetic genistein nanosuspension, in a nonhuman primate model of acute radiation syndrome.

Journal of translational medicine·2026
Same journal

Humanized biparatopic nanobody-based CAR-T cells overcome antigen-heterogeneity in multiple myeloma.

Journal of translational medicine·2026
See all related articles

Related Experiment Video

Updated: Jan 5, 2026

Comprehensive DNA Methylation Analysis Using a Methyl-CpG-binding Domain Capture-based Method in Chronic Lymphocytic Leukemia Patients
13:21

Comprehensive DNA Methylation Analysis Using a Methyl-CpG-binding Domain Capture-based Method in Chronic Lymphocytic Leukemia Patients

Published on: June 16, 2017

10.4K

DNA methylation exploration for ARDS: a multi-omics and multi-microarray interrelated analysis.

Shi Zhang1, Zongsheng Wu1, Jianfeng Xie1

  • 1Department of Critical Care Medicine, Zhongda Hospital, School of Medicine, Southeast University, Nanjing, 210009, China.

Journal of Translational Medicine
|October 19, 2019
PubMed
Summary
This summary is machine-generated.

This study identified 44,439 DNA methylation alterations in acute respiratory distress syndrome (ARDS) patients. Specific DNA methylation sites show potential as therapeutic targets for ARDS treatment.

Keywords:
ARDSDNA methylationInterrelated analysisMulti-omicsmRNA

More Related Videos

Optimized Analysis of DNA Methylation and Gene Expression from Small, Anatomically-defined Areas of the Brain
13:11

Optimized Analysis of DNA Methylation and Gene Expression from Small, Anatomically-defined Areas of the Brain

Published on: July 12, 2012

19.3K
A Rat Methyl-Seq Platform to Identify Epigenetic Changes Associated with Stress Exposure
09:06

A Rat Methyl-Seq Platform to Identify Epigenetic Changes Associated with Stress Exposure

Published on: October 24, 2018

11.2K

Related Experiment Videos

Last Updated: Jan 5, 2026

Comprehensive DNA Methylation Analysis Using a Methyl-CpG-binding Domain Capture-based Method in Chronic Lymphocytic Leukemia Patients
13:21

Comprehensive DNA Methylation Analysis Using a Methyl-CpG-binding Domain Capture-based Method in Chronic Lymphocytic Leukemia Patients

Published on: June 16, 2017

10.4K
Optimized Analysis of DNA Methylation and Gene Expression from Small, Anatomically-defined Areas of the Brain
13:11

Optimized Analysis of DNA Methylation and Gene Expression from Small, Anatomically-defined Areas of the Brain

Published on: July 12, 2012

19.3K
A Rat Methyl-Seq Platform to Identify Epigenetic Changes Associated with Stress Exposure
09:06

A Rat Methyl-Seq Platform to Identify Epigenetic Changes Associated with Stress Exposure

Published on: October 24, 2018

11.2K

Area of Science:

  • Genomics
  • Epigenetics
  • Medical Research

Background:

  • Acute respiratory distress syndrome (ARDS) lacks novel therapeutic targets despite clinical advances.
  • DNA methylation is a reversible process implicated in disease development and a potential therapeutic avenue for ARDS.
  • Understanding specific DNA methylation sites in ARDS is crucial for developing new treatments.

Purpose of the Study:

  • To identify differences in DNA methylation between ARDS patients and healthy individuals.
  • To explore feasible DNA methylation markers for potential therapeutic targets in ARDS.
  • To investigate the relationship between DNA methylation and gene expression in ARDS.

Main Methods:

  • Analysis of microarray data from the GEO database for ARDS and healthy participants.
  • Utilized R packages (limma, VennDiagram, Perl, sva) for data analysis, merging, and heterogeneity reduction.
  • Functional annotation and enrichment analyses (GO, KEGG) to understand the biological significance of methylation sites and genes.
  • Receiver operating characteristic (ROC) curve analysis to identify diagnostic markers.

Main Results:

  • Identified 44,439 DNA methylation alterations and 29 differentially expressed mRNAs between ARDS and healthy controls.
  • Found 40 methylation variations regulating the transcription of 16 genes.
  • Highlighted 30 DNA methylation sites associated with inflammation, immunity, endothelial function, epithelial function, and coagulation.
  • Identified cg03341377, cg24310395, cg07830557, and cg08418670 as potential diagnostic markers with AUC up to 0.99.

Conclusions:

  • Significant DNA methylation alterations and mRNA differences exist between ARDS and healthy controls.
  • 30 DNA methylation sites may regulate 10 genes involved in ARDS pathogenesis, representing potential intervention targets.
  • Further validation experiments are warranted to assess these findings as therapeutic targets for ARDS.