Antibody Phage Display: Antibody Selection in Solution Using Biotinylated Antigens

Area of Science:

• Biotechnology
• Immunology
• Molecular Biology

Background:

• Antibody phage display is a widely used in vitro method for generating recombinant antibodies.
• These antibodies serve critical roles in research, diagnostics, and therapeutics.
• Eleven FDA/EMA-approved therapeutic antibodies, including adalimumab, were developed using phage display by autumn 2018.

Purpose of the Study:

• To describe the antibody selection process, known as panning.
• To highlight the advantages of solution-phase panning over immobilized antigen panning.
• To provide detailed protocols for panning and screening monoclonal antibody binders.

Main Methods:

• Describing the antibody selection process (panning) in solution.
• Comparing solution panning with panning on immobilized antigens.
• Detailing protocols for panning procedures and screening of monoclonal binders.

Main Results:

• Solution-phase panning offers advantages for antibody selection.
• Detailed protocols facilitate the generation and screening of monoclonal binders.
• Successful application of phage display has led to multiple approved therapeutics.

Conclusions:

• The choice of antibody selection method is crucial for successful in vitro generation of human antibodies.
• Solution-phase panning presents a valuable approach for antibody discovery.
• Phage display technology continues to be a powerful tool for developing therapeutic antibodies.