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Updated: Jan 5, 2026

Hemi-laryngeal Setup for Studying Vocal Fold Vibration in Three Dimensions
Published on: November 25, 2017
Estimating vocal fold stiffness: Using the relationship between subglottic pressure and fundamental frequency of
Wen-Hsuan Tseng1,2, Chi-Chin Chang3, Tsung-Lin Yang1,2,4
1Department of Otolaryngology, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei, Taiwan.
Objective:
The stiffness of the vocal folds is an important factor in voice production, yet clinically applicable measurements are still lacking. It has been demonstrated in an in vivo canine model that fundamental frequency (F0 ) increased linearly as subglottic pressure (Ps ) increased, but with a lesser slope for higher levels of vocal fold tension. In this study, the relationship between F0 and Ps was investigated using the airflow interruption method in awake patients non-invasively.
Design:
Healthy volunteers enrolled for evaluation.
Setting:
Single-centre.
Participants:
Thirty-three healthy volunteers aged 20 and older were recruited, with one excluded for a recent asthma attack.
Main Outcome Measures:
The relationships between F0 and Ps , described as the slope (Hz/kPa), were investigated when the participants sustained voicing the vowel/o/at 3 incremental frequencies 4 semitones apart in the modal register (F1, F2 and F3).
Results:
Thirty-two healthy volunteers (20 females, 12 males) aged 20-47 years were enrolled for final analyses. There was a statistically significant difference in the slopes of the linear regression lines of F0 -Ps , depending on the frequency with which the vowel/o/ was produced (P < .001). The slope differed significantly between F2 and F1 (P < .001; P = .015), F3 and F1 (P < .001; P = .002) and F3 and F2 (P < .001; P = .005) for both women and men, respectively.
Conclusions:
It was demonstrated that the higher the vocal fold tension, the smaller the slope between F0 and Ps . Using the relationship between F0 and Ps as an analog of vocal fold stiffness is potentially practical for clinical application.
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