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Updated: Jan 5, 2026

Sampling and Pretreatment of Tooth Enamel Carbonate for Stable Carbon and Oxygen Isotope Analysis
Published on: August 15, 2018
[A method for examining the biochemical composition of dental tissues in human teeth]
B N Izotov1, P S Velenko2, S B Lisovskaya1
1Department of Analytical Toxicology, Pharmaceutical Chemistry and Pharmacognosy, Institute of Pharmacy and Translational Medicine, Sechenov First Moscow State Medical University, Ministry of Health of Russia, Moscow, Russia, 128018.
Abstract:
In medical literature there is no single detailed standard for studying the biochemical composition of a tooth. This circumstance led to the development of a proprietary method for determining the enantiomers of aspartic acid in hard dental tissues for use in the further study of the relationship between the amino acid composition of dental tissues and the biological age of humans. Dental tissue samples obtained from living individuals aged from 20 to 50 years were studied by chromatography-mass spectrometry. The results of the study allowed us to put forward a number of practical recommendations on the standardization of the procedure for the preparation of dental tissue samples and the parameters of chromatography-mass spectrometry research.

