UPLC Orbitrap MS/MS-based fingerprints of dissolved organic matter in waste leachate driven by waste age

Waste leachate is a pool of complicated metabolites from waste treatment and disposal as a global environmental problem. The recognition of dissolved organic matter (DOM) in leachate is crucial to improve leachate treatment efficiency and comprehend waste stabilization process. The present study acquired the molecular information for DOM in 22 waste leachate samples using ultra-performance liquid chromatography coupled with hybrid quadrupole Orbitrap mass spectrometry (UPLC Orbitrap MS/MS) based on two dimensions of retention time and mass-to-charge ratio. Unique mass peaks occupied more than 20% of the detected mass peaks in each leachate, implying that the molecular information for DOM could be the fingerprint of waste landfills and storage pits. Waste age and composition predominately accounted for this unique DOM. The double-bond equivalent increased and the H/C decreased with waste age. We further found that 57 precursor ion peaks and artificial matter (confirmed as N-butylbenzenesulfonamide) were significantly correlated with waste age by multiple test and non-target screening. These molecular characteristics of raw leachate were first determined to compensate for the evolution of leachate with waste age. The fingerprints of waste leachate can be further applied in environmental monitoring scenarios, e.g., tracing landfill leakage.