A New pH-Dependent Macrocyclic Rhodamine B-Based Fluorescent Probe for Copper Detection in White Wine

For efficiently measuring copper (II) ions in the acidic media of white wine, a new chemosensor based on rhodamine B coupled to a tetraazamacrocyclic ring (13aneN₄CH₂NH₂) was designed and synthesized by a one-pot reaction using ethanol as a green solvent. The obtained chemosensor was characterized via NMR, UV and fluorescent spectra. It was marked with no color emission under neutral pH conditions, with a pink color emission under acidic conditions, and a magenta color emission under acidic conditions where copper (II) ions were present. The sensitivity towards copper (II) ions was tested and verified over Ca²⁺, Ag⁺, Zn²⁺, Mg²⁺, Co²⁺, Ni²⁺, Fe²⁺, Pb²⁺, Cd²⁺, Fe³⁺, and Mn²⁺, with a detection limit of 4.38 × 10^-8 M in the fluorescence spectrum.