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Rapid assay for von Willebrand factor activity using formalin-fixed platelets and microtitration technic
American Journal of Clinical Pathology
|December 1, 1979
Summary
A new, inexpensive assay for von Willebrand factor (VWF) was developed using microtitration plates and formalin-fixed platelets. This simple method is suitable for routine diagnostic laboratories to assess patients with prolonged bleeding times.
Area of Science:
- Hematology
- Clinical Diagnostics
Background:
- Von Willebrand factor (VWF) is crucial for hemostasis.
- Deficiencies in VWF can lead to prolonged bleeding times.
- Accurate VWF assays are essential for diagnosing bleeding disorders.
Purpose of the Study:
- To describe a novel, cost-effective assay for quantifying von Willebrand factor.
- To evaluate the suitability of this assay for routine diagnostic use.
Main Methods:
- The assay utilizes microtitration plates.
- Formalin-fixed platelets serve as a key reagent.
- Ristocetin is employed as an activator.
Main Results:
- The described method provides an inexpensive assay for VWF.
- The assay is technically simple to perform.
- It is suitable for use in diagnostic laboratories.
Conclusions:
- This assay offers a practical and affordable option for VWF measurement.
- It can aid in the diagnosis of patients presenting with prolonged bleeding times.