Source: Gao, Z., et al. A Mouse Model of Direct Anastomosis via the Prespinal Route for Crossing Nerve Transfer Surgery. J. Vis. Exp. (2021).This video demonstrates restoring forelimb movement in a mouse via direct cervical nerve reconnection. It begins with a mouse with a paralyzed right forelimb due to a transected right C7 nerve at its nerve root. The left C7 nerve is severed, guided to the right via the prespinal route, and sutured with the right C7 nerve. The surgical site is then cleaned...

Begin with a genetically modified (GM) mouse with disrupted downstream signaling of mGluR5, a neurotransmitter receptor.In the hippocampus of a regular mouse, mGluR5 regulates endocytosis of fast-acting excitatory receptors from existing synapses, weakening them.Internalized receptors are recycled to newly formed synapses, strengthening them for memory formation.In the GM mouse, impaired mGluR5 signaling reduces receptor redistribution, disrupting new memory formation.Expose the mouse to a...

Source: Akil, O., et al., Surgical Method for Virally Mediated Gene Delivery to the Mouse Inner Ear through the Round Window Membrane. J. Vis. Exp. (2015)This video demonstrates the procedure of delivering a therapeutic gene into the inner ear of a mouse pup with genetically induced hearing loss. Using an adeno-associated viral (AAV) vector, the gene is injected through the round window membrane, allowing viral particles to enter hair cells, restore glutamate transporter production, and...

Here, we evaluated the impact of various polishing methods on the color stability of compomer and composite resin restorations used in anterior restorations within pediatric dentistry. This study highlights the significance of selecting appropriate polishing methods to enhance the color stability of compomer and composite resin restorations in pediatric dentistry.

Here, we present a Cas9-based exon23 deletion protocol to restore dystrophin expression in iPSC from Dmdmdx mouse-derived skin fibroblasts and directly differentiate iPSCs into myogenic progenitor cells (MPC) using the Tet-on MyoD activation...

Restorative care is provided once a patient has been discharged from a healthcare facility and requires additional services. The additional services include home care, rehabilitation programs, and extended care. Restorative care centers help the patient regain their previous level of functioning or acquire a new level of functioning due to the incapacitating effects of a disease or a disability. It aims to assist patients in enhancing their quality of life by encouraging independence,...