Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

Next-generation Sequencing

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Nur77 agonism invigorates Natural Killer cell immunity against hepatocellular carcinoma.

Nature communications·2026

Same author

Resolving the Haplotype Complexity of Colorectal Cancer Genomes with Droplet Barcode Sequencing.

Life (Basel, Switzerland)·2026

Same author

Chromatin-associated intronic RNAs from long genes form introsomes that shape nuclear architecture in neuronal cells.

bioRxiv : the preprint server for biology·2026

Same author

Expert recommendations for PIK3CA testing in HR+/HER2- locally advanced and metastatic breast cancer.

NPJ breast cancer·2026

Same author

World Health Organization classification of tumours of the breast 6th edition 2026.

Histopathology·2026

Same author

Genome-wide biomarker analysis across the full spectrum of HER2-expressing breast cancers to reveal a clonal chromosome 17 imbalance defining unfavourable HER2-low disease.

Biomarker research·2026

Same journal

Chlorinated VSLSs Surpass HCFCs in CFC-11-Equivalent Emissions for Ozone Layer Depletion in China.

Nature communications·2026

Same journal

Author Correction: Charge transfer in triphenylamine-tetrazine covalent organic frameworks for solar-driven hydrogen peroxide production.

Nature communications·2026

Same journal

Vegetation browning patterns under compound soil and atmospheric dryness in northern permafrost ecosystems.

Nature communications·2026

Same journal

Voltage imaging of CA1 pyramidal cells and SST+ interneurons reveals stability and plasticity mechanisms of spatial firing.

Nature communications·2026

Same journal

Radical-omics reveals the hydrogen-abstraction pathway of isoprene oxidation.

Nature communications·2026

Same journal

Toughening elastomer via sequentially activated multi-pathway energy dissipation.

Nature communications·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jan 5, 2026

Gel-seq: A Method for Simultaneous Sequencing Library Preparation of DNA and RNA Using Hydrogel Matrices

Gel-seq: A Method for Simultaneous Sequencing Library Preparation of DNA and RNA Using Hydrogel Matrices

Published on: March 26, 2018

CUTseq is a versatile method for preparing multiplexed DNA sequencing libraries from low-input samples.

Xiaolu Zhang¹, Silvano Garnerone¹, Michele Simonetti¹

¹Science for Life Laboratory, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, SE-17165, Sweden.

Nature Communications

|October 20, 2019

Summary

This summary is machine-generated.

CUTseq barcodes and amplifies genomic DNA before library construction, offering a cost-effective and versatile solution for reduced representation genome sequencing. This method improves library preparation for challenging samples like FFPE tissues.

More Related Videos

Ultralow Input Genome Sequencing Library Preparation from a Single Tardigrade Specimen

Ultralow Input Genome Sequencing Library Preparation from a Single Tardigrade Specimen

Published on: July 15, 2018

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies

Published on: April 11, 2016

Related Experiment Videos

Last Updated: Jan 5, 2026

Gel-seq: A Method for Simultaneous Sequencing Library Preparation of DNA and RNA Using Hydrogel Matrices

Gel-seq: A Method for Simultaneous Sequencing Library Preparation of DNA and RNA Using Hydrogel Matrices

Published on: March 26, 2018

Ultralow Input Genome Sequencing Library Preparation from a Single Tardigrade Specimen

Ultralow Input Genome Sequencing Library Preparation from a Single Tardigrade Specimen

Published on: July 15, 2018

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies

Published on: April 11, 2016

Area of Science:

Genomics
Molecular Biology
Biotechnology

Background:

Current massively parallel sequencing relies on costly and time-consuming library indexing.
Existing methods face challenges with fixed samples, such as formalin-fixed paraffin-embedded (FFPE) tissues.

Purpose of the Study:

To introduce CUTseq, a novel method for barcoding and amplifying genomic DNA prior to library construction.
To evaluate the sensitivity and reproducibility of CUTseq.
To demonstrate CUTseq's application in profiling DNA copy number variations in FFPE tumor sections.

Main Methods:

CUTseq utilizes restriction enzymes and in vitro transcription for DNA barcoding and amplification.
The method is applied to both cell lines and FFPE samples.
CUTseq enables multi-region DNA copy number profiling within single FFPE tumor sections.

Main Results:

CUTseq demonstrates high sensitivity and reproducibility in cell lines and FFPE samples.
The method facilitates high-resolution spatial profiling of intratumor genetic heterogeneity.
CUTseq proves effective for reduced representation genome sequencing.

Conclusions:

CUTseq is a versatile and cost-effective method for genomic DNA library preparation.
The technique offers significant advantages for research and diagnostics, particularly with FFPE samples.
CUTseq addresses limitations of current multiplexing strategies in next-generation sequencing.