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Improving Diameter Accuracy for Dynamic Imaging Microscopy for Different Particle Types.

Richard Ernest Cavicchi1, Dean C Ripple1, 1

  • 1National Institute of Standards and Technology, Gaithersburg, Maryland 20899.

Journal of Pharmaceutical Sciences
|October 20, 2019
PubMed
Summary
This summary is machine-generated.

Calibration of dynamic imaging analysis instruments using polystyrene microspheres may skew results for biopharmaceutical particles. New correction factors based on particle type are needed for accurate particle size distribution analysis.

Keywords:
image analysisimaging methodmicroparticlesmicrospheresparticle sizephysical characterizationprotein aggregation

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Area of Science:

  • Biopharmaceutical analysis
  • Particle characterization
  • Materials science

Background:

  • Dynamic imaging analysis instruments are crucial for sizing particles in biopharmaceuticals.
  • Current calibration methods use polystyrene microspheres in water, a system unlike typical biopharmaceutical formulations.
  • Existing instruments apply image processing thresholds and corrections for fuzzy boundaries, potentially impacting accuracy.

Purpose of the Study:

  • To investigate the influence of instrument thresholds and built-in corrections on particle size and distribution.
  • To determine accurate size corrections for dynamic imaging systems by comparing image diameters with Brownian motion tracking.
  • To evaluate the performance of current calibration methods with various particle types relevant to biopharmaceuticals.

Main Methods:

  • Characterized various particles including monoclonal antibody aggregates, bovine serum albumin aggregates, silicone oil droplets, polystyrene microspheres, and ethylene tetrafluoroethylene particles.
  • Determined size corrections by comparing equivalent image diameters from dynamic imaging with diameters obtained via Brownian motion tracking.
  • Analyzed the impact of image processing thresholds and built-in corrections on reported particle size and distribution.

Main Results:

  • Protein aggregates and ethylene tetrafluoroethylene particles exhibited similar characteristics, differing significantly from polystyrene calibration spheres.
  • The study revealed that instrument thresholds and built-in corrections influence reported particle size and distribution.
  • Significant discrepancies were observed between polystyrene calibration and actual biopharmaceutical particle types.

Conclusions:

  • Current calibration procedures using polystyrene microspheres are inadequate for accurate sizing of diverse biopharmaceutical particles.
  • Developing new, particle-type-specific correction factors and calibration methods is essential for reliable dynamic imaging analysis.
  • This research paves the way for improved accuracy in biopharmaceutical particle characterization.