Diesel exhaust particle and dust mite induced airway inflammation is modified by cerium dioxide nanoparticles

Cerium dioxide nanoparticles (CeO₂NPs) have been used as diesel fuel-borne catalysts for improved efficiency and pollutant emissions. Concerns that such material may influence diesel exhaust particle (DEP) effects within the lung upon inhalation, prompted us to examine particle responses in mice in the presence and absence of the common allergen house dust mite (HDM). Repeated intranasal instillation of combined HDM and DEP increased airway mucin, eosinophils, lymphocytes, IL-5, IL-13, IL-17A and plasma IgE, which were further increased with CeO₂NPs co-exposure. A single co-exposure of CeO₂NPs and DEP after repeated HDM exposure increased macrophage and IL-17A levels above DEP induced levels. CeO₂NPs exposure in the absence of HDM also resulted in increased levels of plasma IgE and airway mucin staining, changes not observed with repeated DEP exposure alone. These observations indicate that CeO₂NPs can modify exhaust particulate and allergen induced inflammatory events in the lung with the potential to influence conditions such as allergic airway disease.