A label-free and carbon dots based fluorescent aptasensor for the detection of kanamycin in milk

A novel label-free aptasensor for kanamycin detection was constructed using gold nanoparticles (AuNPs) as absorber to quench the fluorescence of carbon dots (CDs) via the inner filter effect (IFE). The strategy was mainly relied on the fact that the absorption spectra of AuNPs overlapped with the fluorescence excitation spectra of fluorophores as well as the specific binding capacity of Ky2 aptamer to kanamycin. Upon adding kanamycin antibiotic, the free aptamer sequences are firstly exhausted to form some complexes, which leads to AuNPs aggregation in high salt concentration. Consequently, the absorber's absorption spectrum changes and no longer overlaps with the fluorescence emission spectrum of the CDs, which results in obvious fluorescence recovery of the aptasensor. Herein, the effects of some vital parameters like the type and number of nanoparticles on the fluorescent aptasensor have been investigated. Under optimal conditions, the proposed aptasensor can detect kanamycin in a linear range of 0.04-0.24 μM, with a limit of detection (LOD) as low as 18 nM. Moreover, the further studies also validate the applicability of the proposed aptasensor in milk samples, revealing that it may have enormous potential utility for practical kanamycin detection in food products in the future.