Micromonospora orduensis sp. nov., isolated from deep marine sediment

A novel actinobacterial strain, designated S2509^T, was isolated from marine sediment collected by a dredge at a depth of 45 m along Melet River offshore of the southern Black Sea coast, Ordu, Turkey. The cell wall peptidoglycan of strain was found to contain meso-diaminopimelic acid and 3-OH-diaminopimelic acid. The whole cell sugars detected were arabinose, glucose, rhamnose, ribose and xylose. The diagnostic phospholipids of strain S2509^T were found to be diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, a glycolipid and two unidentified phospholipids. The predominant menaquinones were identified as MK-9(H₈), MK-9(H₆), MK-10(H₈), MK-9(H₄), MK-10(H₄) and MK-10(H₆). The major cellular fatty acids were found to be iso-C_16:0, iso-C_15:0 and 10-methyl C_17:0. The taxonomic position of the strain was established using a polyphasic approach, showing that S2509^T strain belongs to the genus Micromonospora. Phylogenetic analysis based on the 16S rRNA gene sequence of strain S2509^T showed that it is closely related to the type strain of Micromonospora chokoriensis DSM 45160^T (99.37% sequence similarity), and phylogenetically clustered with Micromonospora inaquosa LB39^T (99.37%), Micromonospora lupini Lupac 14N^T (99.16%), Micromonospora violae NEAU-zh8^T (99.23%) and Micromonospora taraxaci NEAU-P5^T (99.03%). The phylogenetic analysis based on the gyrB gene sequence of strain S2509^T confirmed its close relationship with M. chokoriensis JCM 13247^T (96.5% sequence similarity). Whole genome sequences confirmed by digital DNA-DNA hybridization analysis that the strain S2509^T represents a novel species in the genus Micromonospora, for which the name Micromonospora orduensis sp. nov. is proposed. The type strain is S2509^T (=DSM 45926^T = KCTC 29201^T).