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Updated: Jan 5, 2026
Experimental Protocol for Using Drosophila As an Invertebrate Model System for Toxicity Testing in the Laboratory
Published on: July 10, 2018
Exploring the multilevel hazards of thiamethoxam using Drosophila melanogaster
Xiaoqin Li1, Jinyue Liu1, Xing Wang1
1College of Resources and Environmental Sciences, China Agricultural University, Beijing, 100193, China; Beijing Key Laboratory of Biodiversity and Organic Farming, Beijing, 100193, China.
Abstract:
Thiamethoxam (THIA) is a widely used pesticide. However, its effects on the growth and development of insects remain unclear. Herein, the lethal concentration 50 (LC50) of THIA (3.13 μg/mL for adults, 20.25 μg/mL for third-instar larvae) were identified. THIA (1/3 LC50) prolonged the time required for growth and development, and decreased the fecundity, the rates of pupation and eclosion, and lifespan of Drosophila. The uniform architecture of the compound eyes was disturbed. It also triggered DNA damage, and reduced the viability of fat body cells and hemocytes. Moreover, RNA-sequencing showed that differentially expressed genes in response to THIA were mainly related to stratum corneum development, immune function. Genes involved in stratum corneum proteins (Lcp65Ag3, Cpr65Ax1), hemocyte proliferation (RyR), and immune responses (IM4) were significantly induced. Genes associated with lipid metabolism (sxe2), lifespan (Atg7 and NalZ), pupa development (IIp8, Blimp-1), female fertility (Ddc), male mating behavior (ple), neural retina development (Nnad), was significantly downregulated. These findings provide a basis for further research to fully assess the hazards of exposure to neonicotinoid pesticides.
