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Updated: Jan 5, 2026

Measuring Attachment and Internalization of Influenza A Virus in A549 Cells by Flow Cytometry
Published on: November 4, 2015
A flow cytometric granularity assay for the quantification of infectious virus
Megan Logan1, Julia Manalil1, Christina Notte2
1Department of Chemical Engineering, University of Waterloo, Waterloo, Ontario N2L3G1, Canada.
Abstract:
A flow cytometry-based assay was developed to assess the infective titer of two recombinant viruses: a recombinant herpes simplex type 2 (rHSV-2) and a recombinant canary pox (rALVAC.gfp). This method uses granularity of infected Vero and QT-35 cells, respectively, and correlates this to the infectious titer of virus samples. The percent of the cell populations with a high level of granularity could accurately be correlated to viral titers obtained through a traditional plaque assay, with R2 values greater than 0.8 using a semi-logarithmic scale. This approach offers a rapid, high-throughput method for infectious virus titration with similar accuracy to a traditional plaque assay.

