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Updated: Jan 5, 2026

A Preclinical Mouse Model of Osteosarcoma to Define the Extracellular Vesicle-mediated Communication Between Tumor and Mesenchymal Stem Cells
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Silencing of synaptotagmin 7 regulates osteosarcoma cell proliferation, apoptosis, and migration

Zhiqiang Wu1,2, Zhengwang Sun1,2, Rui Huang3

  • 1Department of Oncology, Shanghai Medical College, Fudan University, Xuhui District, Shanghai, China.

Histology and Histopathology
|October 22, 2019
PubMed
Abstract

Insights

Synaptotagmin 7 (SYT7) is upregulated in osteosarcoma and promotes cancer cell growth, proliferation, and metastasis. Silencing SYT7 inhibits tumor development, suggesting it as a potential therapeutic target for osteosarcoma.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Synaptotagmin 7 (SYT7) is a key protein in the synaptotagmin family involved in various physiological and pathological processes.
  • Its specific role in osteosarcoma, a primary bone cancer, requires detailed investigation.

Purpose of the Study:

  • To elucidate the expression patterns of SYT7 in osteosarcoma tissues.
  • To determine the functional significance of SYT7 in osteosarcoma cell behavior, including proliferation, cell cycle, apoptosis, migration, and invasion.

Main Methods:

  • Immunohistochemistry was used to quantify SYT7 expression in tumor and adjacent tissues.
  • Statistical analyses (Mann-Whitney U, Spearman correlation) assessed the relationship between SYT7 levels and clinicopathological features.
  • In vitro assays (MTT, flow cytometry, wound healing, Transwell) evaluated the impact of SYT7 knockdown on osteosarcoma cell functions.

Main Results:

  • SYT7 expression was significantly elevated in osteosarcoma tissues compared to para-sarcoma tissues.
  • Higher SYT7 levels correlated positively with adverse pathological characteristics of osteosarcoma.
  • SYT7 silencing markedly suppressed cell proliferation, colony formation, migration, and invasion.
  • Knockdown of SYT7 induced G2 phase cell cycle arrest and promoted apoptosis.

Conclusions:

  • SYT7 plays a critical role in the pathogenesis and progression of osteosarcoma.
  • The findings highlight SYT7 as a promising novel diagnostic biomarker and therapeutic target for osteosarcoma treatment.

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