Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Studying the Cytoskeleton01:17

Studying the Cytoskeleton

8.4K
The cytoskeletal architecture can be studied using different microscopic and biochemical techniques. Electron microscopy was instrumental in discovering the cytoskeletal architecture around the 1960s, which allowed obtaining structural information at a high-resolution level. However, the sample preparation procedure often limits this ability in biological samples. Several protocols have been developed over the years to optimize sample preparation. In one of the protocols known as rotary...
8.4K
Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

5.2K
Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.
5.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Saturated anacardic acid shows promise as adjuvant therapy for preventing severe malaria via attenuation of inflammatory response by modulation of the NF-κB pathway.

Acta tropica·2026
Same author

Microplastic contamination of donated blood and red cell concentrates.

British journal of haematology·2026
Same author

Antimicrobial Activity and Characterization of a Validated Copper-Complexed Polymer Tape for Surface Disinfectant Applications.

Antibiotics (Basel, Switzerland)·2025
Same author

ZnO nanoparticles enhance the cytotoxic effects of a chalcone-sulfonamide hybrid by impairing the autophagic flux and upregulating HIF-1α expression.

Bioorganic chemistry·2025
Same author

The Importance of Feedstock and Process Control on the Composition of Recovered Carbon Black.

Polymers·2025
Same author

Enhanced apoptosis, inflammatory cellularity, collagen deposition, and interaction between fibroblasts and Leishmania amastigotes in undamaged ear skin of dogs with leishmaniosis.

Veterinary parasitology·2025

Related Experiment Video

Updated: Jan 5, 2026

Traction Force Microscopy to Study B Lymphocyte Activation
09:28

Traction Force Microscopy to Study B Lymphocyte Activation

Published on: July 23, 2020

6.7K

Atomic Force Microscopy Is a Potent Technique to Study Eosinophil Activation.

Peter Eaton1,2, Constança Pais do Amaral1, Shirley C P Couto3

  • 1Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Lisbon, Portugal.

Frontiers in Physiology
|October 22, 2019
PubMed
Summary

Atomic force microscopy (AFM) reveals distinct morphological changes in eosinophils during acute asthma exacerbations. This sensitive technique can detect functional alterations in these cells, aiding disease evaluation.

Keywords:
acute asthmaadvanced microscopyallergic diseasesatomic force microscopycell morphological changeseosinophils

More Related Videos

Functionalization of Atomic Force Microscope Cantilevers with Single-T Cells or Single-Particle for Immunological Single-Cell Force Spectroscopy
10:06

Functionalization of Atomic Force Microscope Cantilevers with Single-T Cells or Single-Particle for Immunological Single-Cell Force Spectroscopy

Published on: July 10, 2019

7.8K
Contact Mode Atomic Force Microscopy as a Rapid Technique for Morphological Observation and Bacterial Cell Damage Analysis
05:34

Contact Mode Atomic Force Microscopy as a Rapid Technique for Morphological Observation and Bacterial Cell Damage Analysis

Published on: June 30, 2023

2.3K

Related Experiment Videos

Last Updated: Jan 5, 2026

Traction Force Microscopy to Study B Lymphocyte Activation
09:28

Traction Force Microscopy to Study B Lymphocyte Activation

Published on: July 23, 2020

6.7K
Functionalization of Atomic Force Microscope Cantilevers with Single-T Cells or Single-Particle for Immunological Single-Cell Force Spectroscopy
10:06

Functionalization of Atomic Force Microscope Cantilevers with Single-T Cells or Single-Particle for Immunological Single-Cell Force Spectroscopy

Published on: July 10, 2019

7.8K
Contact Mode Atomic Force Microscopy as a Rapid Technique for Morphological Observation and Bacterial Cell Damage Analysis
05:34

Contact Mode Atomic Force Microscopy as a Rapid Technique for Morphological Observation and Bacterial Cell Damage Analysis

Published on: June 30, 2023

2.3K

Area of Science:

  • Cell Biology
  • Biophysics
  • Immunology

Background:

  • Eosinophils play diverse roles in health and disease.
  • Morphological changes and increased numbers of eosinophils correlate with acute asthma severity.

Purpose of the Study:

  • To investigate eosinophil morphology in healthy controls versus acute asthma patients using atomic force microscopy (AFM).
  • To compare the sensitivity of AFM with traditional microscopy for detecting eosinophil alterations.

Main Methods:

  • Eosinophils were isolated from healthy individuals and patients with acute asthma.
  • Atomic force microscopy (AFM) was employed to image and analyze cell morphology.
  • Morphological features, including cell shape, spreading, pseudopod emission, and granule degranulation, were assessed.

Main Results:

  • Healthy eosinophils exhibited rounded, partially spread morphologies.
  • Asthma-related eosinophils showed increased spreading and multiple pseudopod emissions.
  • Increased eosinophil degranulation was observed in asthma samples compared to controls.
  • AFM provided significantly more detailed morphological insights than brightfield microscopy.

Conclusions:

  • AFM is a highly sensitive tool for detecting eosinophil morphological changes indicative of functional alterations.
  • AFM can differentiate between healthy and asthma-associated eosinophil morphologies.
  • This technique holds potential for evaluating diseases involving eosinophil dysfunction.