Related Experiment Video
Updated: Jan 5, 2026
Application of Laparoscopic Hepatectomy Combined with Intraoperative Microwave Ablation in Colorectal Cancer Liver Metastasis
Published on: March 3, 2023
Effects of microbubble-enhanced ultrasound combined with prothrombin on microwave ablation in rabbit VX2 liver tumor
Ying Shi1,2, Ping Yan3, Zhenli Luo4
1Department of Ultrasound, The Second Hospital of Shanxi Medical University Taiyuan 030001, Shanxi, China.
Objective:
This study aimed to investigate effects of microbubble-enhanced ultrasound (MEUS) combined with prothrombin on microwave ablation (MWA) on VX2 liver tumors in a rabbit model.
Methods:
80 rabbits with VX2 liver tumors were randomly divided into Group A (sham + NS), Group B (sham + NS + P), Group C (MEUS), and Group D (PMEUS). After treatment, targeted liver tumors were ablated with MWA. On 0, 3, 7 and 14 d, volume of coagulated area was measured. Tissues in ablated area, transition area and surrounding area were collected.
Results:
On 0, 3, 7 and 14 d, coagulated volume in Group D was larger than remaining three groups (P<0.05). On 7 d and 14 d, tumor volume in Group D was smaller than remaining three groups (P<0.05). Fibrotic band in Group D was wider than in remaining three groups (P<0.05). Cellular ultrastructure injury in ablated area on 0 d and mitochondrial injury in transition area on 7 d were more severe in Group D than in remaining three groups. On 0, 3, 7 and 14 d, proliferative cellular nuclear antigen-positive index in transition area in Group C and Group D was lower than Group A and Group B (P<0.05). On 0, 3 and 7 d, apoptosis index in transition area in Group D was higher than remaining three groups (P<0.05).
Conclusions:
MEUS combined with prothrombin on MWA can significantly expand ablation volume, enhance the necrosis of ablated tissues, inhibit tumor growth/metastasis and improve therapeutic effect of MWA on rabbit VX2 liver tumors.
