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Related Concept Videos

Protein Networks02:26

Protein Networks

4.4K
An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
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Protein-protein Interfaces02:04

Protein-protein Interfaces

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Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...
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Identification of Novel Proteins Interacting with Proprotein Convertase Subtilisin/Kexin 9.

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Atorvastatin and lovastatin, but not pravastatin, increased cellular complex formation between PCSK9 and the LDL receptor in human hepatocyte-like C3A cells.

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Hypercholesterolemia: The role of PCSK9.

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Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions
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Identification of Proteins Interacting with PCSK9 Using a Protoarray Human Protein Microarray.

Catherine J Wooten1, Sreevidhya T Krishnaji2, Quantil M Melendez1

  • 1Department of Pharmaceutical Sciences, Biomanufacturing Research Institute and Technology Enterprise (BRITE), College of Arts and Sciences, North Carolina Central University, Durham, NC 27707, USA.

International Journal of Biomedical Investigation
|October 22, 2019
PubMed
Summary
This summary is machine-generated.

Researchers identified 15 novel proteins interacting with proprotein convertase subtilisin-kexin 9 (PCSK9). This discovery offers potential for personalized medicine in treating hypercholesterolemia by understanding PCSK9

Keywords:
HypercholesterolemiaMetabolic diseasesPCSK9Protein-protein interactionProtoarray

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Genetics

Background:

  • Proprotein convertase subtilisin-kexin 9 (PCSK9) plays a role in various biological processes.
  • Understanding PCSK9 interactions is crucial for managing hypercholesterolemia.

Purpose of the Study:

  • To identify novel proteins that interact with PCSK9.
  • To explore potential therapeutic targets for hypercholesterolemia.

Main Methods:

  • Utilized a ProtoArray Human Protein Microarray.
  • Employed biotinylated PCSK9 for interaction studies.

Main Results:

  • Identified 15 novel proteins interacting with PCSK9.
  • Sterol carrier protein 2 and hepatoma-derived growth factor, related protein 3 were among the identified proteins with known functions.

Conclusions:

  • The identified proteins may influence PCSK9 expression or function.
  • These findings hold promise for personalized medicine approaches in hypercholesterolemia treatment.