A Simple Approach to Perform TEER Measurements Using a Self-Made Volt-Amperemeter with Programmable Output Frequency

Area of Science:

• Biomedical Engineering
• Cell Biology
• Electrophysiology

Background:

• Transepithelial/endothelial electrical resistance (TEER) is a standard method for assessing in vitro barrier model integrity and permeability.
• Current TEER measurement typically employs specialized devices with chopstick electrodes, which can be costly.
• Existing TEER techniques are applied across diverse cell models, including blood-brain barrier, gastrointestinal, and pulmonary systems.

Purpose of the Study:

• To design and implement a more affordable and adaptable TEER measurement system.
• To create a device with programmable output frequency for enhanced experimental control.
• To enable the use of commercially available chopstick electrodes with a self-assembled voltammeter.

Main Methods:

• Self-assembly of a custom voltammeter for electrical impedance measurements.
• Integration of programmable output frequency capabilities into the device.
• Utilization of standard laboratory filter insert systems and commercially available chopstick electrodes.

Main Results:

• A functional and reliable TEER measurement device was successfully constructed.
• The custom device demonstrated compatibility with standard laboratory equipment and electrodes.
• Programmable frequency output allows for nuanced analysis of barrier properties.

Conclusions:

• A cost-effective and customizable TEER measurement system can be self-assembled.
• This approach offers a viable alternative to commercially available TEER devices.
• The developed system provides flexibility for various in vitro barrier model applications.