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Related Experiment Video

Updated: Jun 25, 2026

Competitive Genomic Screens of Barcoded Yeast Libraries

Competitive Genomic Screens of Barcoded Yeast Libraries

Published on: August 11, 2011

Beyond protein binding: recent advances in screening DNA-encoded libraries.

Thomas Kodadek¹, Nicholas G Paciaroni¹, Madeline Balzarini¹

¹Department of Chemistry, The Scripps Research Institute, 130 Scripps Way, Jupiter, FL 33458, USA. kodadek@scripps.edu.

Chemical Communications (Cambridge, England)

|October 22, 2019

Summary

DNA-encoded library (DEL) screening is a key technique for discovering drug molecules. Novel DEL assay formats now enable more complex screening beyond simple binding, including selectivity, enzyme inhibition, and organocatalyst identification.

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Last Updated: Jun 25, 2026

Competitive Genomic Screens of Barcoded Yeast Libraries

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Published on: August 11, 2011

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Kinetic Screening of Nuclease Activity using Nucleic Acid Probes

Kinetic Screening of Nuclease Activity using Nucleic Acid Probes

Published on: November 1, 2019

Area of Science:

Medicinal Chemistry
Chemical Biology
Drug Discovery

Background:

DNA-encoded library (DEL) screening is a powerful technology for identifying small molecule ligands.
Traditional DEL screening primarily relies on simple binding assays against protein targets.
Recent advancements have expanded DEL capabilities beyond basic binding interactions.

Purpose of the Study:

To review novel DNA-encoded library (DEL) screening formats.
To highlight DEL applications beyond simple binding assays.
To discuss current challenges and future directions in DEL assay development.

Main Methods:

Incubation of DELs with target molecules (typically proteins).
Washing to remove non-specific binders.
Amplification and deep sequencing of DNA tags to identify bound ligands.
Implementation of specialized assay formats for selectivity, enzyme inhibition, and organocatalysis.

Main Results:

Demonstration of DELs' utility in complex screening paradigms.
Successful application of DELs for identifying selective binders, enzyme inhibitors, and organocatalysts.
Identification of new assay formats expanding the scope of DEL technology.

Conclusions:

DEL screening is evolving beyond simple binding assays.
Novel DEL formats offer enhanced capabilities for drug and probe discovery.
Addressing remaining challenges is crucial for further advancing DEL technology.