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Updated: Jan 5, 2026

Visualization of the Immunological Synapse by Dual Color Time-gated Stimulated Emission Depletion STED Nanoscopy
Published on: March 24, 2014
A super-resolution platform for correlative live single-molecule imaging and STED microscopy
V V G Krishna Inavalli1,2, Martin O Lenz1,2, Corey Butler1,2,3
1Interdisciplinary Institute for Neuroscience, University of Bordeaux, Bordeaux, France.
Abstract:
Super-resolution microscopy offers tremendous opportunities to unravel the complex and dynamic architecture of living cells. However, current super-resolution microscopes are well suited for revealing protein distributions or cell morphology, but not both. We present a super-resolution platform that permits correlative single-molecule imaging and stimulated emission depletion microscopy in live cells. It gives nanoscale access to the positions and movements of synaptic proteins within the morphological context of growth cones and dendritic spines.
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