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The flicker Pupil Light Response (fPLR).

Prakash Adhikari1,2, Beatrix Feigl1,3,4, Andrew J Zele1,2

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Translational Vision Science & Technology
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Summary

The flicker pupil light response (fPLR) is driven by melanopsin and rod photoreceptors. This study quantifies their contributions across light levels and wavelengths, aiding standardized measurements.

Keywords:
coneflicker pupil light responsemelanopsinphotoreceptorrodspectral sensitivity

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Area of Science:

  • Vision Science
  • Photoreceptor Physiology
  • Neuroscience

Background:

  • The specific photoreceptor classes influencing the flicker pupil light response (fPLR) to monochromatic sinusoidal temporal modulation remain largely undetermined.
  • Understanding these inputs is crucial for interpreting pupil responses and their underlying neural pathways.

Purpose of the Study:

  • To identify and quantify the contributions of different photoreceptor classes to the fPLR.
  • To investigate the influence of irradiance and wavelength on the fPLR.

Main Methods:

  • Measured the 0.5-Hz fPLR in healthy observers across a wide range of irradiances (scotopic to photopic) and five narrowband wavelengths using a Maxwellian view pupillometer.
  • Derived relative photoreceptor contributions from amplitude-irradiance functions.

Main Results:

  • fPLR amplitude increased with irradiance following a Hill function, with wavelength-dependent saturation.
  • Melanopsin and rod photoreceptors were identified as the primary inputs, with their relative weighting changing with light conditions.
  • fPLR phase delay decreased linearly with increasing irradiance.

Conclusions:

  • The fPLR serves as a marker for melanopsin and rod interactions under flicker stimulation.
  • These findings enable quantification of photoreceptor contributions to the post-illumination pupil response (PIPR).
  • The established irradiance and wavelength responses will facilitate standardization of fPLR measurements using chromatic pupillometry.